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11.
The purpose of this study was to understand the variation in root litter mass‐loss rates in a climatic transect across a large region ranging from the Arctic Circle (66°N) in Scandinavia to Berlin (52°N) in north‐east Germany, dominated by coniferous monocultural forests. To investigate the combined influence of litter quality and climate, data were used from 37 sites and plots at which root litters of three coniferous species (Pinus sylvestris, Pinus contorta and Picea abies) had been incubated. In spite of the considerable climatic differences among sites, there were no strong relationships between any climatic variable and the lst‐yr mass loss (range 17.0–40.9%). For the lst‐yr mass loss, the average annual temperature was the most rate‐regulating factor for all litter combined, with a value for R 2 adj of 0.186. Substrate quality also had an influence. Thus, for the whole region lignin concentration was significant, with a value for R 2 adj of 0.142. For the separated groups of pine and spruce stands the value for r 2 increased but the factor average temperature still dominated and for pine the R 2 adj reached a value of 0.346. Over the region N concentration in the fresh pine root litter was significant (R2 adj = 0.232). For the root litter of P. abies the average temperature in July was the strongest rate‐regulating climatic factor, with a value for R 2 adj of 0.381. A combination of temperature in July and the initial P concentration in the litter gave an R 2 adj value of 0.713 for P. abies litter, thus explaining about 71% of the variation.  相似文献   
12.
Shoot tips excised from in vitro cultured plants of Dianthus caryophyllus L. (cv. Pallas, cv. Pink Candy and cv. Wanessa) were successfully cryopreserved using an encapsulation-vitrification method. Shoot tips (2–3 mm in length) were encapsulated in sodium alginate, precultured on liquid Murashige and Skoog (1962) medium supplemented with various sucrose concentrations (0.25, 0.5, 0.75, 1.0 M) for 24 h or 48 h and dehydrated with the vitrification solution PVS2 (up to 4 h) at 24 °C or 0 °C prior to direct immersion in liquid nitrogen (−196 °C). A maximum of shoot regeneration from cryopreserved shoot tips was obtained with the following combinations: preculture in 0.5 M sucrose and 180 min dehydration treatment at 0 °C for cv. Pallas (60% shoot formation), or preculture in 0.75 M and 200 min dehydration at the same temperature for cv. Pink Candy (66.6% shoot formation) and cv. Wanessa (73% shoot formation).  相似文献   
13.
    
Nitrate, ammonium, total dissolved nitrogen (TDN), dissolved organic nitrogen (DON) and dissolved organic carbon (DOC) concentrations and flux were measured for one year in bulk deposition and throughfall from three stand types (upland deciduous, upland conifer and wetland conifer) on the Boreal Plain, Canada. Annual (November 2006 to October 2007 water year) flux rates in bulk deposition were 80, 216, 114 and 410 mg N m−2 for nitrate, ammonium, DON and TDN, respectively, and 3.5 g C m−2 for DOC. The nitrate and ammonium flux in throughfall were approximately 50% of the flux in bulk deposition, while TDN flux in throughfall was 60–74% of the flux in bulk deposition. The DOC flux in throughfall was approximately 2 times greater than DOC flux in bulk deposition, while there was no detectable difference in DON flux. The forest canopy generally had the most impact on throughfall chemistry during the active growing season as compared with the dormant season, although DOC concentrations in throughfall of deciduous stands was highest during autumn. For the upland stands, TDN flow-weighted mean concentrations in the snowpack were not detectably different from the concentrations in throughfall and bulk deposition throughout the rest of the year. However, ammonium concentrations were lower and DON concentrations were higher in the snowpack than in either throughfall or bulk deposition for the other seasons, suggesting some transformation of ammonium to DON within the snowpack.  相似文献   
14.
 We studied the relationship between urease activity (UA) and soil organic matter (SOM), microbial biomass N (Nbiom) content, and urea-N fertilizer assimilation by maize in a Dark Red Latosol (Typic Haplustox) cultivated for 9 years under no-tillage (NT), tillage with a disc plough (DP), and tillage with a moldboard plough (MP). Two soil depths were sampled (0–7.5 cm and 7.5–15 cm) at 4 different times during the crop cycle. Urea was applied at four different rates, ranging from 0 to 240 kg N ha–1. The levels of fertilizer N did not affect the UA, SOM content, and Nbiom content. No significant difference between the treatments (NT, DP, and MP) was observed for SOM during the experiment, probably because the major part of the SOM was in recalcitrant pools, since the area was previously cultivated (conventional tillage) for 20 years. The Nbiom content explained 97% and 69% of the variation in UA in the upper and deeper soil layer, respectively. UA and biomass N were significantly higher in the NT system compared to the DP and MP systems. The highest maize productivity and urea-N recovery was also observed for the NT system. We observed that the increase in urea-N losses under NT, possibly as a consequence of a higher UA, was compensated for by the increase in N immobilized in the biomass. Received: 2 July 1999  相似文献   
15.
    
Lowland evergreen rainforests in southern Chile growing on highly productive soils and accessible sites have been subjected to traditional and industrial logging of valuable timber trees. Old-growth rain forests in this area are characterized by highly conservative N cycles, which results in an efficient N use of ecosystems. We hypothesize that different logging practices, by changing forest structure and species composition, can alter the quantity and quality (i.e. C/N ratio) of litterfall and soil organic matter and soil microbial processes that determine N storage and availability. To test this hypothesis we investigated chemical properties, microbial N transformations, N fluxes and N storage in soils of lowland evergreen rainforests of Chiloé Island after 10 years since industrial selective logging (ISL) and in stands subjected to traditional selective logging (TSL) by landowners in small properties. We compared them to reference unlogged old-growth stands (OG) in the same area. Tree basal area was more reduced in the stands subjected to ISL than to TSL. Litterfall inputs were similar in both logging treatments as in OG stands. This was due to greater biomass of understory species after logging. In TSL understory tree species determined a higher litterfall C/N ratio than ISL. We found higher soil N availability and content of base cations in surface soils of logged forests than in OG. The litter horizon of OG forest had significantly higher rates of non-symbiotic N fixation than logged forests. In the ISL treatment there was a trend toward increasing soil denitrification and significantly higher NO3–N/Nt ratio in spring waters, which led to a stronger δ15N signal in surface and deep soils. We conclude that massive understory occupation by the shade-intolerant native bamboo Chusquea quila in ISL led to enhanced litter quality (lower C/N ratios) relaxing the tightness of the N cycle, which increased soil N availability leading to a higher proportion of nitrate in spring waters and higher gaseous N losses. In contrast, under TSL a higher litterfall C/N ratio slowed decomposition and net N mineralization rates thus reducing the chances for N losses, and enhancing C and N storage in soil. We suggest that sustainable logging practices in these rain forests should be based on lower rates of canopy removal to enhance colonization of the understory by shade-tolerant trees, which are associated with a more efficient N cycle.  相似文献   
16.
    
A better understanding of the fate of fertilizer nitrogen (N) is critical to design appropriate N management strategies in plastic-mulched croplands. We evaluated the effects of plastic mulch on urea-N recovery by crops and loss from soil in furrow-ridge plots, with and without maize (Zea mays L.) cropping, in a semi-arid rain-fed site in China. We applied the same rate of urea-N (281 kg ha−1) to all treatments during the preparation of the furrow-ridges in 2011 and 2012 but 15N-labeled the urea in 2011 only. We used transparent film to cover all soil surfaces in the mulched treatments and seeded maize in furrows in treatments with crop. In 2011, plastic mulch increased the total N uptake in the aboveground biomass of maize by 53%, whereas it decreased the in-season labeled-N uptake by 19%, compared to non-mulched treatment. At harvest in 2011, in mulched treatments the total labeled-N remaining in the 0−170 cm soil layer was 25% greater whereas unaccounted labeled-N was 69% less, than in non-mulched treatments, regardless of whether maize was cropped. In 2012 the effect of mulch on total maize N uptake was comparable to that in 2011, but the residual soil labeled-N uptake by maize was 63% higher in mulched compared to non-mulched treatment. At harvest in 2012, plastic mulch increased total labeled-N remaining in the 0−170 cm depth in cropped soils and unaccounted labeled-N in non-cropped soils, compared with no mulch. Our results indicate that plastic mulch profoundly changes the fate of urea-N in maize production in cold and dry croplands.  相似文献   
17.
    
Soybean (Glycine max (L.) Merr.) is able to fix atmospheric nitrogen in symbiosis with the bacteria Bradyrhizobium japonicum. Because these bacteria are not native in European soils, soybean seeds must be inoculated with Bradyrhizobium strains before sowing to fix nitrogen and meet their yield potential. In Central Europe soybean cultivation is still quite new and breeding of early maturing soybean varieties adapted to cool growing conditions has just started.Under these low temperature conditions in Central Europe the inoculation with different, commercially available Bradyrhizobium inoculants has resulted in unsatisfactory nodulation. The aim of this study was: (i) to test the ability of commercially available inoculants to maximize soybean grain yield, protein content and protein yield, (ii) to study the interaction of different inoculants with different soybean varieties for two different sites in Germany under cool growing conditions over three years and (iii) to determine the variability of biological nitrogen fixation. Field trials were set up on an organically managed site at the Hessische Staatsdomäne Frankenhausen (DFH) and on a conventionally managed site in Quedlinburg (QLB) for three consecutive seasons from 2011 to 2013. Three early maturing soybean varieties—Merlin, Bohemians, Protina—were tested in combination with four different Bradyrhizobium inoculants—Radicin No.7, NPPL-Hi Stick, Force 48, Biodoz Rhizofilm—and compared with a non-inoculated control. Effective inoculation with Bradyrhizobium strains increased grain yield, protein content and protein yield by up to 57%, 26% and 99%, respectively. Grain yield, protein content and protein yield were generally higher in DFH. Average grain yield was 1634 kg ha−1 in QLB (2012–2013) and 2455 kg ha−1 in DFH (2011–2013), average protein content was 386 g kg−1 in QLB and 389 g kg−1 in DFH and average protein yield was 650 kg ha−1 in QLB and 965 kg ha−1 in DFH. The percentage of nitrogen derived from air (Ndfa) ranged between 40% and 57%. Soybeans inoculated with Radicin No. 7 failed to form nodules, and crop performance was identical to the non-inoculated control. Biodoz Rhizofilm, NPPL Hi-Stick and Force 48 are suitable for soybean cultivation under cool growing conditions in Germany. Interactions between soybean variety and inoculant were significant for protein content and protein yield at both sites, but not for nodulation, grain yield, thousand kernel weight and Ndfa. The variety Protina in combination with the inoculant Biodoz Rhizofilm can be recommended for tofu for both tested sites, while Merlin and Protina in combination with Biodoz Rhizofilm are recommended for animal fodder production in DFH. Animal fodder production was not profitable in QLB due to low protein yields.  相似文献   
18.
    
Crop residue is often grazed by sheep after harvest, over the dry summer period from December to March in Mediterranean environments. However, soil cover provided by crop residues is a key component of conservation agriculture for maintaining favourable soil structure and high yields.A series of 31 site × year experiments was conducted to assess the effect of summer stubble grazing on residue levels and following crop yields. Relatively light grazing, with stocking rates below 10 dry sheep equivalent (DSE) and between 90 and 471 DSE days ha−1, had no significant effect on the amount of residue, soil properties, soil water, weeds or yield in the following crop. The main effect of grazing was to knock down and scatter the standing crop residues. However, longer term grazing at relatively high intensity (956 DSE days ha−1) on heavy soil, over both summer and winter, as in a pasture phase, did significantly reduce residue levels, infiltration and yield (by 59%). The effect of summer grazing on soil mineral N was small and inconsistent, with increased mineral N, by about 3–7 kg N ha−1, following grazing at two of the 13 sites. By contrast, higher mineral N, by 2–15 kg N ha−1, was measured in the un-grazed plots at three of the 13 sites. This was due to increased growth of legume pastures in the absence of grazing.More research is needed to confirm the yield effects when cropping after an annual pasture/fallow that is grazed over summer and winter, particularly on different soil types.  相似文献   
19.
    
Salinity stress and inefficient nitrogen fertilization adversely affect cotton growth and yield. The effect of salinity on the growth and stress response of cotton seedlings and the effect on N‐use efficiency from the use of the inhibitors of urease (NBPT) and nitrification (DCD) under salinity stress were studied in growth chambers. The study consisted of three levels of salinity – low (0.45 dS m?1), moderate (8 dS m?1) and high (16 dS m?1) – and five N treatments – unfertilized control, 100 % N rate with urea, 80 % N rate with urea, 80 % N rate with urea +NBPT and 80 % N rate with urea +NBPT + DCD. The results indicated that salinity stress reduced plant growth (low leaf area and plant dry matter), decreased N assimilation (low NR, GS and protein), increased plant stress response (high GR and SOD), and decreased leaf chlorophyll, stomatal conductance and quantum yield. Addition of NBPT to urea improved N uptake by 22 % under low salinity; however, this effect was not observed with increasing salinity. No benefit of addition of DCD was observed in any of the parameters collected. In conclusion, salinity stress hindered the performance of the additive NBPT and negatively affected the growth and physiology of cotton.  相似文献   
20.
    
Algarrobo (Prosopis alba) is an important nitrogen-fixing tree adapted to the semiarid regions of northwestern Argentina. The tree provides fuelwood and dimensionally stable lumber for flooring and furniture; its pods that have a high sugar content are consumed by livestock and humans. P. alba has been one of the most heavily harvested species. This paper reports on the evaluation of a nine-year-old Prosopis alba progeny trial containing 57 half-sibling families of eight provenances in northwestern Argentina (of the 1,596 trees planted, 1,289 survived in 1999). Considering the multiple uses of P. alba, the selection criteria included: total biomass production (from basal diameter using regression equations), height, rate of pod production, and pod sensory characteristics. The family-narrow-sense-heritability was 0.487 for height. 0.548 for biomass production, and 0.244 for pod production. In 1998, 12 of the 1,289 trees were more than 4 m tall and had more than 1.75 kg pods per tree; and their pods had a sweet or very sweet non-astringent taste. Scions from these 12 trees were successfully grafted onto unselected P. alba rootstock. These are currently being used to produce rooted cuttings. The pod production, biomass, and height of the 12 clones ranged from 6.55 to 14.4, 1.57 to 13.5, and 1.29 to 1.70 times the respective population means. The genetic gains of 13.5%, 77%, and 147% for height, biomass, and pod production respectively are greater than genetic gains for other tree species and are probably due to the great genetic variability in the local population and the lack of any prior genetic improvement program.This revised version was published online in November 2005 with corrections to the Cover Date.  相似文献   
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